45 search results for buffer

Is IDTE Buffer RNase-free?

Yes, IDTE Buffer is nuclease-free.

We test for both DNases and RNases using our DNaseAlert and RNaseAlert Kits.

Is IDTE Buffer RNase-free?

Yes, IDTE Buffer is nuclease-free.

We test for both DNases and RNases using our DNaseAlert and RNaseAlert Kits.

Can 1X TE buffer be used instead of IDTE Buffer in rhAmpSeq™ experiments?

No. 1X TE buffer is typically 10 mM Tris, 1 mM EDTA, while IDTE (1X TE solution) is 10 mM Tris, 0.1 mM EDTA. The additional EDTA in 1X TE buffer can...

Can 1X TE buffer be used instead of IDTE Buffer in rhAmpSeq™ experiments?

No. 1X TE buffer is typically 10 mM Tris, 1 mM EDTA, while IDTE (1X TE solution) is 10 mM Tris, 0.1 mM EDTA. The additional EDTA in 1X TE buffer can...

What buffer should I use to dilute the xGen™ Stubby Adapter for Element?

We recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, catalog # 10006743) to dilute the xGen Stubby Adapter for Element.

Keeping the adapters duplexed requires a buffer with a ...

What buffer should I use to dilute the xGen™ Stubby Adapter?

We recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, Cat. No. 10006743) to dilute the xGen Adapters...

What buffer should I use to dilute the xGen™ Stubby Adapter for DNBSEQ™?

We recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, Cat. No. 10006743) to dilute the xGen Stubby Adapter for DNBSEQ. Keeping the adapters duplexed requires a buffer with a high salt ...

What buffer should I use to dilute the xGen™ Stubby Adapter, xGen™ PCR-Free Adapters for Ultima, and xGen™ HybCap Adapters for Ultima?

We recommend using a buffer made of 10 mM Tris, 0.1 mM EDTA, and 100 mM NaCl, pH 8.0 (such as the IDT NGS Adapter Buffer, Cat. No. 10006743) to dilute the xGen™ Stubby Adapter, xGen™ PCR-Free Adapters for Ultima, and xGen™ HybCap Adap...

What buffer should I use to resuspend the Cas12a protein?

All Cas12a variants are provided as a 10 mg/mL solution and, therefore, resuspension is not required.

What buffer should I use to resuspend the Cas9 protein?

All Alt-R™ Cas9 nucleases and nickases are provided in solution at 10 mg/mL, so resuspension is not necessary.

Can I anneal my DNA oligos at room temperature and if so, what buffer should I use?

Although it may be possible to anneal oligos at room temperature, heating to denature the oligos and then cooling slowly to anneal the two oligos will help to ensure more efficient annealing and favor the stable duplex formation.

If you choose ...

What conditions should I use to HPLC purify my oligos?

IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for an RP-HPLC protocol is:

• Hamilton PRP-1 Reverse Phase column 250 x 10 mm
• Buffer A = 100% ACN (acetonitrile)
• Buffer B = ACN/0.1 M TEAA pH 7.3
• Run at 4 mL/min over 30 min from 95–60% A
• Monitor at 260/297 nm

Are the RUO and GMP products the same?

Yes, both products are the same protein construct and the same formulation buffer to ensure equivalent performance. Specifications are very similar between the two products. 

What are optimum storage conditions for a custom gene?

IDT ships custom gene dry unless otherwise requested. While dry they should be stable up to 2 years. After resuspending in a high-quality, molecular-grade water or buffer, pH 7.5–8.0...

How do I resuspend my oligos?

We find it convenient to initially make a 100 µM freezer stock, which should be thawed relatively infrequently to make more dilute aliquots for short term use. The oligo can be dissolved in TE buffer...

How do I calculate the melting temperature of oligos in salt solutions other than NaCl?

As long as you know the concentration of the monovalent cations in your solution (Na⁺, K⁺, etc.), you can use our free OligoAnalyzer™ Tool to calculate the T_m of your oligo...

What can cause under-fragmentation of DNA?

Under digestion is caused by either improper fragmentation time—see the CoA or product box for recommended lot-specific fragmentation time for the supported insert sizes; incomplete mixing of the master mix before or when combined with the sample...

How should I resuspend the Alt-R HDR Donor Block?

Follow these steps to resuspend Alt-R™ HDR Donor Blocks:

1. Before opening the tube, spin in a microcentrifuge for 3–5 sec to ensure the Alt-R HDR Donor Blocks DNA is in the bottom of the tube.
2. Add molecular grade water, or a buffer such as IDTE, to a final stock concentration of
   500 ng/µL, or a concentration appropriate for your planned experiment.
3. Briefly vortex.
4. Incubate at approximately 50°C for 20 min. Heating the tube will ensure the water or buffer contacts the tiny pellet, even if it is stuck to the side of the tube. Thus, this step increases the likelihood that the entire pellet will be resuspended.
5. Vortex and centrifuge for 3–5 sec. Due to low resuspension volumes required for this product, thorough vortexing or pipetting is recommended to ensure the sides of the tube have been thoroughly exposed to your buffer of choice.
6. Verify the final concentration using the NanoDrop™ (Thermo Fisher Scientific), Qubit® instruments (Thermo Fisher Scientific), or another spectrophotometer. Depending on your analysis method, you may want to make a dilution of your sample for the concentration check to conserve material.

Can I order my oligos resuspended to a 100 µM concentration?

Yes. IDT offers a service called the LabReady service on standard products. With the LabReady service, we ship your oligos at a 100 µM  concentration in IDTE Buffer...

Should I do anything to prep my tube of oligo before resuspending?

We recommend briefly centrifuging your tubes of dried oligo prior to opening them.

This will ensure that the oligo pellet is at the bottom of the tube and will not be lost when you open the cap. After adding non-DEPC treated water or buffer...

What is the shelf life of my oligo?

The shelf life of an oligo is dependent on the temperature at which the oligo is stored and how the oligo is resuspended. Temperature is the more important of the 2 variables. Generally, oligos should be stored at –20°C. At this temper...

What conditions should I use to HPLC purify my oligos?

IDT uses our own proprietary HPLC method to purify oligos in-house. A published set of conditions for an RP-HPLC protocol is:

• Hamilton PRP-1 Reverse Phase column 250 x 10 mm
• Buffer A = 100% ACN (acetonitrile)
• Buffer B = ACN/0.1 M TEAA pH 7.3
• Run at 4 mL/min over 30 min from 95–60% A
• Monitor at 260/297 nm

What are optimum storage conditions for a gBlocks™ HiFi Gene Fragment?

IDT ships gBlocks HiFi Gene Fragments (IDT) dry unless otherwise requested. While dry, they should be stable almost indefinitely.

A...

Should RNA be stored differently than DNA?

The inherent chemical structure of RNA makes it less stable than DNA. RNases that degrade RNA are also more prevalent than DNases.

As RNA is a great deal more sensitive to degradation compared to DNA, for short term storage we recommend using...

Should I only be concerned about sodium ions when calculating melting temperature (T_m)?

Any charge will play a role in the T_m value for an oligonucleotide in a reaction. Thus, sodium, magnesium, and potassium concentrations each contribute to this calculation.

The IDT OligoAnalyzer™ Tool...

What is the shelf life of my oligo?

The shelf life of an oligo is dependent on the temperature at which the oligo is stored and how the oligo is resuspended. Temperature is the more important of the 2 variables. Generally, oligos should be stored at –20°C. At this temper...

What is the concentration of the Alt-R™ Cas9 nucleases and nickases?

The Alt-R Cas9 nucleases and nickases are supplied at concentration of 10 mg/mL in 25 mM Tris-Cl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol (v/v), pH 7.4, with the...

Is it OK to resuspend and store oligos in DEPC treated water?

No, we do not recommend resuspending or storing oligos in DEPC treated water. DEPC treated water can be acidic which may cause depurination of the oligo resulting in degradation.

We recommend that oligos are stored long term at at ‒20°C. ...

Can I order my oligos resuspended to a 100 µM concentration?

Yes. IDT offers a service called the LabReady service on standard products. With the LabReady service, we ship your oligos at a 100 µM  concentration in IDTE Buffer...

What amount of xGen™ UDI-UMI Adapters should I use for library prep?

The amount of xGen UDI-UMI Adapters you use depends on the library prep workflow and the amount of starting sample used to perform library prep. We recommend c...

What can cause over fragmentation of DNA samples?

Improper fragmentation time is one cause of over fragmentation; see the CoA or the product box for the recommended lot-specific fragmentation time for supported fragment sizes.

Over digestion is another cause a...

Is it OK to resuspend and store oligos in DEPC treated water?

No, we do not recommend resuspending or storing oligos in DEPC treated water. DEPC treated water can be acidic which may cause depurination of the oligo resulting in degradation.

We recommend that oligos are stored long term at at ‒20°C. ...

What is the stability of fluorescently labeled oligos and their fluorescent modifications?

The stability of fluorescently labeled oligos and fluorophore function is similar to the stability of a standard oligo.

IDT recommends oligos that are to be stored long term should be stored frozen at –20°C. If the oligos are going ...

What are the optimum storage conditions for Alt-R HDR Donor Blocks?

IDT ships Alt-R™ HDR Donor Blocks dry at ambient temperatures. While dry, we recommend long term storage at -20°C. After resuspending in a high quality, molecular-grade water or buffer, pH 7.5-8.0...

Should I do anything to prep my tube of oligo before resuspending?

We recommend briefly centrifuging your tubes of dried oligo prior to opening them.

This will ensure that the oligo pellet is at the bottom of the tube and will not be lost when you open the cap. After adding non-DEPC treated water or buffer...

Should I only be concerned about sodium ions when calculating melting temperature (T_m)?

Any charge will play a role in the T_m value for an oligonucleotide in a reaction. Thus, sodium, magnesium, and potassium concentrations each contribute to this calculation.

The IDT OligoAnalyzer™ Tool...

How do I calculate the melting temperature of oligos in salt solutions other than NaCl?

As long as you know the concentration of the monovalent cations in your solution (Na⁺, K⁺, etc.), you can use our free OligoAnalyzer™ Tool to calculate the T_m of your oligo...

Can the type of modification incorporated into a DNA or RNA oligo affect the stability or shelf life of the oligo?

For the modifications tested at this point, the pattern of degradation seen for modified oligos is similar to that for standard oligos. However, stability or shelf-life information has not been collected for all modifications offered. 

For...

What reagents will I need in addition to the Alt-R™ guide RNAs to do a CRISPR genome editing experiment?

The Alt-R CRISPR-Cas9 System provides custom CRISPR crRNA and a universal CRISPR tracrRNA, both of which are required for CRISPR-Cas9 genome editing using the Alt-...

What are the ideal storage conditions for promoting long term oligo stability?

For long term oligo storage, temperature is the most important factor to consider. For long term storage, whether oligos are dried down, or resuspended in non-DEPC ...

Should RNA be stored differently than DNA?

The inherent chemical structure of RNA makes it less stable than DNA. RNases that degrade RNA are also more prevalent than DNases.

As RNA is a great deal more sensitive to degradation compared to DNA, for short term storage we recommend using...

What are optimum storage conditions for a gBlocks™ Gene Fragment?

IDT ships gBlocks Gene Fragments dry unless otherwise requested. While dry, they should be stable up to two years.

After resuspend...

What is the stability of fluorescently labeled oligos and their fluorescent modifications?

The stability of fluorescently labeled oligos and fluorophore function is similar to the stability of a standard oligo.

IDT recommends oligos that are to be stored long term should be stored frozen at –20°C. If the oligos are going ...

What reagents will I need in addition to the Alt-R™ RNAs to do a CRISPR genome editing experiment?

The Alt-R CRISPR-Cas9 System provides custom CRISPR crRNA and a universal CRISPR tracrRNA, both of which are required for CRISPR-Cas9 genome editing using the Alt-...

Do the positive and negative controls in the TriFECTa kit come with primers and RT-PCR materials?

The kit does not include primers or PCR reagents for quantification of gene silencing. Separately, we offer primer sets for targeting human or mouse HPRT for use in SYBR® Green–based qPCR assays...