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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How much xGen UDI-UMI Adapters should I use for library prep?

The amount of xGen UDI-UMI Adapters you use depends on the library prep workflow and the amount of starting sample used to perform library prep. We recommend checking your specific protocol for the optimal amount of adapter to use.

For the Lotus DNA Library Prep Kit, use 4 µL of xGen UDI-UMI Adapters (diluted according to the table below) for the ligation reaction.

DNA input (ng) Adapter dilution Stock concentration (µM)
≥25 No dilution 15
10 10-fold (1:10) 1.5
1 20-fold (1:20) 0.75