High-fidelity, single-stranded DNA fragments
Megamer Single-Stranded DNA Fragments are sequence-verified, single-stranded DNA strands of length 201–2000 bases. They can be used in research applications such as homology-directed repair of CRISPR-mediated genome editing, in vitro transcription, and more.
NEW! Try our Alt-R™ HDR Donor Blocks, double-stranded DNA fragments specifically built for your homology-directed repair (HDR) experiments. IDT dsDNA templates offer a cost-effective, high-fidelity option to reduce the amount of downstream screening via higher HDR rates and lower unintended blunt integrations. Alt-R HDR Donor Blocks are ideal for making large insertions or genomic changes.
|Megamer® ssDNA Fragments—Sense + Antisense, 201–500 bases||$790.00 USD|
|Megamer® ssDNA Fragments—Sense + Antisense, >500 bases||$1.70 USD / base|
Includes your ssDNA fragment of interest, without the complementary strand, delivered dry. Fragments are normalized to 3 µg final yield.
|Megamer® ssDNA Fragment, 201–500 bases||$595.00 USD|
|Megamer® ssDNA Fragment, >500 bases||$1.40 USD / base|
Megamer ssDNA Fragments are single-stranded genomic blocks for research applications such as homology-directed repair of CRISPR-mediated genome editing, in vitro transcription, and more. Megamer fragments are 201–2000 bases in length and are generated from with clonally purified DNA, which offers the greatest purity available. Megamer ssDNA Fragments are sequence-verified via next-generation sequencing and are typically shipped within 20 business days*. They are composed of A, T, G, and C nucleotides only. Sequence information is always secure and confidential at IDT. Non-disclosure agreements are available through IDT legal services upon request.
* The time required to manufacture a Megamer ssDNA Fragment is dependent on many factors and may exceed the estimated delivery time.
Integrated DNA Technologies screens every Megamer ssDNA Fragment for potentially dangerous pathogen sequences, and to verify that IDT’s customers are legitimate scientists engaged in beneficial research.
IDT is among the five founding members of the International Gene Synthesis Consortium (IGSC), and helped to create the IGSC’s Harmonized Screening Protocol. The Harmonized Screening Protocol describes the gene sequence and customer screening practices that IGSC member companies employ to prevent the misuse of synthetic genes. IDT takes the steps set out in the Harmonized Screening Protocol to screen the sequences of ordered genes and the prospective customers who submit those orders.
For more information about the IGSC and the Harmonized Screening Protocol, please visit their website.
In October 2010, the United States government issued final Screening Framework Guidance for Providers of Synthetic Double-Stranded DNA, describing how commercial providers of synthetic genes should perform gene sequence and customer screening. IDT and the other IGSC member companies supported the adoption of the Screening Framework Guidance, and IDT follows that Guidance in its application of the Harmonized Screening Protocol. For more information, please see 75 FR 62820 (Oct. 13, 2010), or https://federalregister.gov/a/2010-25728.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.