Processing
HotPlates® DNA Oligos
Custom DNA oligos in plates, delivered in 2 business days
HotPlates DNA Oligos are standard, desalted DNA oligos delivered in V-bottom plates and shipped by our fastest method. Place your order before the specified deadline to receive your plates in 2 business days.
- Receive your custom HotPlate DNA Oligos 2 business days after placing your order
- Each oligo is verified by ESI mass spectrometry*—expedited without compromising quality
- Every oligo normalized to your specifications—let us do the formulation for you
- No maximum on
number of plates
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
HotPlates DNA Oligos must be ordered online and are no longer available by email request.
Product details
HotPlates DNA Oligos are standard, desalted DNA oligos delivered in V-bottom plates and shipped by our fastest method. If you place your order before the day’s deadline (below), you will receive your plates in 2 business days.
Every DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI mass spectrometry methods*, and quantified twice by UV spectrophotometry for accurate yield measurements.
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
Specifications
- Normalized to 2 to 10 nmol per well or provided at full synthesis yield
- 15–60 DNA bases; no modifications or modified bases
- Deprotected and desalted
- Each oligo quality controlled by ESI mass spectrometry*
- Delivered dry in 96-well V-bottom plates
- Minimum of 48 oligos per plate
- Available in US, Canada, and Europe
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
Ordering deadlines
US and Canada
Order online by 3:00 pm ET
Europe
Order online by 1:00 pm CET
Quality
IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.
An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes achieve extremely high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2) to provide you with most consistent quality in the industry. No matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in an extremely reliable product that will perform the same each time it is used. Because your research requires a steady supply of oligos over time, this reliability provides confidence in your research.
Figure 1. Proportion of full-length product is a factor of coupling efficiency. DNA oligos from IDT are sysnthesized on proprietary platforms that deliver higher coupling efficiency than industry standards. The increasing importance of coupling efficiency to increased oligo length is shown.
Figure 2. Daily monitoring of coupling efficiency at all IDT manufacturing sites. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents). We track coupling efficiency at all of our manufacturing sites to ensure consistent quality with change in variables. A 30-day rolling average is shown.
Resources
User guides and protocols
Brochures and flyers
Frequently asked questions
Tools
OligoAnalyzer™ tool
Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
UNAFold tool
Perform secondary structure analysis
Dilution Calculator
Find the volume needed to dilute your oligo stock to a lower concentration
Resuspension Calculator
Find the volume needed to resuspend a dry oligo to a desired concentration
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