HotPlates^® DNA Oligos

HotPlates DNA Oligos are standard, desalted DNA oligos delivered in V-bottom plates and shipped by our fastest method. If you place your order before the day’s deadline (below), you will receive your plates in 2 business days.

Every DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI mass spectrometry methods*, and quantified twice by UV spectrophotometry for accurate yield measurements.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Specifications

• Normalized to 2 to 10 nmol per well or provided at full synthesis yield
• 15–60 DNA bases; no modifications or modified bases
• Deprotected and desalted
• Each oligo quality controlled by ESI mass spectrometry*
• Delivered dry in 96-well V-bottom plates
• Minimum of 48 oligos per plate
• Available in US, Canada, and Europe

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Ordering deadlines

IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.

An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes achieve extremely high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2) to provide you with most consistent quality in the industry. No matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in an extremely reliable product that will perform the same each time it is used. Because your research requires a steady supply of oligos over time, this reliability provides confidence in your research.

User guides and protocols

Brochures and flyers

Frequently asked questions

OligoAnalyzer™ tool

Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches

UNAFold tool

Perform secondary structure analysis

Dilution Calculator

Find the volume needed to dilute your oligo stock to a lower concentration

Resuspension Calculator

Find the volume needed to resuspend a dry oligo to a desired concentration