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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do I determine the Tm of my sequence?

IDT offers a free online calculator, the OligoAnalyzer tool, that will determine the Tm of your oligonucleotide sequence, for reaction conditions you specify. Access it at

Be sure to include the Mg2+ and dNTP concentrations you plan to use in your reaction, as this will affect the Tm for your oligo.

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