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RNA Oligonucleotides

All single-stranded and duplexed RNA sequences are produced using proprietary technology that delivers high quality RNA. Our specialized synthesis platforms allow us to produce the custom high-fidelity guides for CRISPR, duplexes for RNAi, template switching oligos for NGS, microRNAs, aptamers, and custom RNA oligos for other applications.

Ordering

Single-stranded RNA

Shipped dry.

ProductLengthRNA bases2' O-Methyl RNA basesChimeric DNA basesRNase-Free HPLC
100 nmole RNA Oligo10–60 bases$9.50 USD/base$14.00 USD/base$0.84 USD/base$97.00 USD
250 nmole RNA Oligo5–60 bases$12.50 USD/base$22.00 USD/base$1.47 USD/base$133.00 USD
1 umole RNA Oligo5–60 bases$26.00 USD/base$33.00 USD/base$3.11 USD/base$206.00 USD
5 umole RNA Oligo5–60 bases$76.00 USD/base$131.00 USD/base$12.00 USD/base$439.00 USD
10 umole RNA Oligo5–60 bases$113.00 USD/base$241.00 USD/base$20.50 USD/base$754.00 USD

Duplexed RNA

2 oligos, annealed and delivered in a single tube. Shipped dry.

ProductLengthRNA bases2' O-Methyl RNA basesChimeric DNA basesRNase-Free HPLC
100 nmole RNA Duplex Oligo10–60 bases$9.50 USD/base$14.00 USD/base$0.84 USD/base$97.00 USD
250 nmole RNA Duplex Oligo5–60 bases$12.50 USD/base$22.00 USD/base$1.47 USD/base$133.00 USD
1 umole RNA Duplex Oligo5–60 bases$26.00 USD/base$33.00 USD/base$3.11 USD/base$206.00 USD

Annealing fees will be applied to duplexed oligos (per duplex), as follows:

100 nmol:
$39.00 USD
250 nmol:
$51.00 USD
1 µmol:
$58.00 USD

Single-stranded RNA

Shipped dry or resuspended to your specifications. A minimum of 24 or 96 oligos required for 96- or 384-well plates, respectively.

ProductLengthRNA bases2' O-Methyl RNA basesChimeric DNA basesRNase-Free HPLC
100 nmole RNA Plate Oligo10–60 bases$9.50 USD/base$14.00 USD/base$0.38 USD/base$97.00 USD
250 nmole RNA Plate Oligo5–60 bases$12.50 USD/base$22.00 USD/base$0.69 USD/base$133.00 USD
1 umole RNA Plate Oligo5–60 bases$26.00 USD/base$33.00 USD/base$1.32 USD/base$206.00 USD

Product Details

 

RNA oligos are short, single- or double-stranded synthetic RNA sequences that can be used in nearly any RNA­ specific molecular biology application. Using the Oligo Entry ordering tool, you can design your sequences to contain unmodified RNA bases, 2'-O-methyl RNA bases, or chimeric DNA bases. You can select from hundreds of modifications to suit your specific research needs. (2'-O-methyl RNA is a useful RNA analog that is resistant to degradation by single-stranded ribonucleases. Every RNA Oligo you receive is deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled using ESI mass spectrometry* and quantified by UV spectrophotometry to provide yield measurements.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Using the RNA Oligo Entry tool

  • To enter individual RNA bases, insert "r" before the base (rA, rU, rC, rG)
  • To enter individual 2′-O-Methyl RNA bases, insert "m" before the base (mA, mU, mC, mG)
  • To quickly convert an entire oligo to RNA, enter your sequence as DNA using A, T, C, G and then click “Convert to “RNA”. All bases in your sequence will be converted to RNA (rA, rU, rC, rG)

Quality

IDT's reputation as a pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation. We rarely rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents. Our ability to control these variables allows us to produce high-quality oligos.

The inherent structure of RNA makes it more difficult to manufacture than DNA. However, our proprietary nucleic acid synthesis technology allows us to obtain high coupling efficiencies, meaning you will receive RNA oligos with the yield and purity that your project needs.

Services

The following services represent our most common offerings. If your project requires a service not listed here, contact us and we will work with you to accommodate your request.

Purification

For more demanding RNA applications, additional purification by RNase-Free HPLC purification is needed. Most purified oligos up to 60 bases receive a purity guarantee* following quality control by capillary electrophoresis or analytical HPLC. Learn more »

ProductRNase-Free HPLC
100 nmole RNA Oligo$97.00 USD
250 nmole RNA Oligo$133.00 USD
1 umole RNA Oligo$206.00 USD
5 umole RNA Oligo$439.00 USD
10 umole RNA Oligo$754.00 USD

*Based on sequence characteristics

Formulation

Diverse application requirements and individual researcher preferences necessitate oligos that can be delivered in unique formats. You can have your oligos prepared to your specific requirements. Learn more »

For information on available services, contact us.

Custom quality control

In addition to the free ESI mass spectrometry analysis that is provided for your oligos*, your project may require one of the more advanced analytical services listed below.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

ProductAnalytical IE-HPLC pH 12.0Analytical RP-HPLC
100 nmole RNA Oligo$72.00 USD$72.00 USD
250 nmole RNA Oligo$72.00 USD$72.00 USD
1 umole RNA Oligo$72.00 USD$72.00 USD
5 umole RNA Oligo$72.00 USD$72.00 USD
10 umole RNA OligoN/A$72.00 USD
Description96 well Plate384 well Plate
Entire Plate Capillary Electrophoresis$512.00 USD$2,060.00 USD

Large-scale synthesis

Custom RNA oligos can be ordered in bulk with the same high quality, competitive pricing, and fast turnaround times you expect from IDT. To learn more about our large-scale synthesis capabilities, click here.

GMP and OEM

If you require oligos that are ISO 13485 certified, or if you are interested in our third-party manufacturing services, please click here.

Frequently asked questions

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