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oPools™ Oligo Pools

Start with solid bases

Use oPools Oligo Pools for accurate, reliable, and affordable CRISPR libraries; primer pools for multiplex PCR; gene construction; data storage; and FISH analysis. These pools of custom single-stranded DNA sequences offer high fidelity, uniformity, low error rates, and low dropout rates. This means that you can avoid amplification bias, varying concentrations, or high error rates that are often encountered when using pooled oligos from other suppliers.


oPools Oligo Pools

Pooled DNA oligos delivered dry in a single tube.

Available in convenient sizes:

Scale (pmol/oligo)Number of oligos per poolOligo length (bases)
1100 to 20,00040 to 350
1010 to 200040 to 350
502 to 38440 to 350

* Regardless of pool size, the first 3300 bases will be charged at the oPools DNA Base 1 rate. Bases 3301–50,000 will be charged at the oPools DNA Base 2 rate, bases 50,001–100,000 will be charged at the oPools DNA Base 3, and bases more than 100,000 will be charged at the oPools Base 4 rate.

The following service fees will be applied for modifications:
Price per oligo
5' Phosphorylation


Product details

oPools Oligo Pools are single-stranded DNA sequences that are used for CRISPR library construction, primer pools for multiplex PCR, gene construction, data storage, and FISH analysis.

Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramer™ DNA Oligos. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, oPools DNA Oligos use a unique solid support specifically optimized to synthesize high-quality oligos up to 350 bases in length.


Standard processing
Length 40–350 bases/oligo
Scale (number of oligos)
1 pmol/oligo(100 to 20,000 oligos/pool)
10 pmol/oligo(10 to 2000 oligos/pool)
50 pmol/oligo(2 to 384 oligos/pool)
Mixed bases
N = A, C, G, and T
K = G and T
• Limited to 10 mixed bases per oligo
Modifications 5′ phosphorylation
QC and quantification None
Shipping container 2 mL tube

Shipping and storage

  • Shipped dry
  • Turnaround time: 4–7 business days from order to delivery
  • Short-term storage, up to 6 weeks: room temperature (15 to 25°C) or 4°C
  • Long-term storage, up to 24 months: –20°C

Product data

Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids.

IDT offers better coupling efficiency than other suppliers

Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.6% (IDT oligos, n = 34,627) and 99.4% (other supplier, n = 77 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.

Proprietary DNA synthesis equipment produces an even oligo yield distribution.

Figure 2. Proprietary DNA synthesis results in an even yield distribution of oPools Oligo Pools. This even yield distribution is shown here as a function of % difference from the mean. The standard deviation observed across ½ million sequences is less than 23% of the mean, demonstrating a high level of uniform sequence representation.

oPools Oligo Pools demonstrate low dropout rates

Dropout rate refers to the likelihood that any individual sequence is not present in the final pool. This rate can vary by length and sequence complexity. The average dropout rate for oPools oligos across all length ranges was 0.8% based on a sample size of over 900,000 oligos.

Frequently asked questions