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xGen™ Acute Myeloid Leukemia (AML) Cancer Hybridization Panel

Predesigned panel that targets AML-associated genes

The xGen Acute Myeloid Leukemia (AML) Cancer Hyb Panel comprises 11,731 xGen Hyb Probes, spanning 1.19 Mb of the human genome, for research using targeted enrichment of >260 AML-associated genes, for more efficient identification of disease-causing mutations in research samples.

xGen NGS—made for acute myeloid leukemia research.

Ordering

  • 98% of genomic targets covered at greater than 0.2X mean coverage
  • Detect variations
  • Empirically derived targets
  • Enjoy fast turnaround via easy online ordering and next day shipping

For the xGen Hybridization and Wash Kit, xGen Universal Blockers, xGen Library Amplification Primer Mix, and/or xGen Human Cot DNA, please visit the xGen Hybridization Capture Core Reagents page.

Product details

The xGen AML Cancer Hyb Panel is composed of individually synthesized and quality controlled xGen Hyb Probes that have a 5’ biotin group for capture with streptavidin-coated beads. The panel consists of 11,731 xGen Hyb Probes targeting empirically derived regions within 260 acute myeloid leukemia (AML)-associated genes.

Panel composition (target genes)

Table 1: Frequently researched genes included in the xGen AML Cancer Hyb Panel. *

 ASXL1 FAM154B IDH2 KDR NPM1 PRAMEF2
 C17orf97 FAM47A JAK1 KIT NRAS PTPN11
 CBL FAM5C JAK2 KRAS NTRK3 RUNX1
 CEBPA FLRT2 JAK3 LRRC4 OR13H1 TET2
 DNMT3A FLT3 KCNA4 MLL3 OR8B12 TP53
 EGFR GJB3 KCNK13 MYC P2RY2 TUBA3C
 EZH2 IDH1 KDM6A NF1 PCDHB1 WT1

 

* The panel does not target every exon in each gene, but targets all regions found to be mutated in the TCGA study [1].

Product data

Coverage uniformity for the xGen AML Cancer Hyb Panel

Figure 1. Coverage uniformity obtained with xGen  AML Cancer Panel. Greater than 0.2X mean coverage is observed for >98% of targets. Illumina TruSeq® HT libraries (n=8) were enriched using the xGen AML Cancer Panel and sequenced on a NextSeq 550 system using 2 x 150 paired-end reads. Each sample was sub-sampled to 4M total reads. 

Figure 2. Deep coverage of targeted regions using xGen AML Cancer Panel. Eight genomic DNA libraries were enriched using the xGen Acute Myeloid Leukemia Cancer Panel and sequenced on a NextSeq 550 system using 2 x 150 paired-end reads. Total reads for each sample were sub-sampled to 4M total reads. In all samples, there was >1X coverage for 99.3% of targets and >30X coverage for 98.7% of targets.

Consistent results between lots

Figure 3. Consistent results obtained with xGen AML Cancer Panel.  Genomic DNA libraries were enriched with two lots of the xGen AML Cancer Panel (n=8 for each lot). The enriched samples were sequenced on the NextSeq 550 sequencing platform using 2 x150 paired-end reads. All samples were sub-sampled to a level of 4M total reads. The probe-by-probe target coverage was averaged for eight replicate samples for each lot of the xGen AML Cancer panel. A comparison of probe-by-probe target coverage between two lots show consistent results between lots, with an R2 value of 0.9887.

References

  1. Cancer Genome Atlas Research N, Ley TJ, Miller C, et al. Genomic and epigenomic landscapes of adult de novo acute myeloid leukemia. N Engl J Med. 2013;368(22):2059-2074.
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