Yes. The master mix has been successfully used in qPCR experiments run on the QuantStudio™ 7 Flex (Thermo Fisher Scientific), 7900HT (Thermo Fisher Scientific), 7500 (Thermo Fisher Scientific), the CFX384™ (BioRad), and the LightCycler® 480 (Roche) instruments. All such experiments used the cycling conditions provided in the protocol,
PrimeTime Gene Expression Master Mix.
Reference dye is provided in a separate tube, so you may add it to the master mix at the levels required by your real-time PCR instrument.