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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What amount of xGen™ UDI-UMI Adapters should I use for library prep?
The amount of xGen UDI-UMI Adapters you use depends on the library prep workflow and the amount of starting sample used to perform library prep. We recommend checking your specific protocol for the optimal amount of adapter to use.
For the xGen DNA Library Prep Kits, use 5 µL of xGen UDI-UMI Adapters (diluted according to the table below) for the ligation reaction.
Note: If diluting the xGen Stubby Adapter is required, we recommend using Nuclease-Free Duplex Buffer.
| DNA input (ng) | Adapter dilution | Stock concentration (µM) |
|---|---|---|
| ≥25 | No dilution | 15 |
| 10 | 10-fold (1:10) | 1.5 |
| 1 | 20-fold (1:20) | 0.75 |
Reach out to our Scientific Applications Support team if you are unsure.
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.