Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How much cDNA is recommended per PrimeTime qPCR Assay reaction?

For optimal performance of the assays, use 1 to 100 ng cDNA per 20 µL reaction.