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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How many freeze-thaw events can the PrimeTime™ One-Step 4X Broad-Range qPCR Master Mix withstand?
We tested 5 true freeze-thaw cycles and no detrimental effects or loss in Cq were observed. The PrimeTime™ One‑Step 4X Broad‑Range qPCR Master Mix is not expected to freeze at –20°C, so 20 use-case scenarios were also tested and once again no detrimental effects or loss in Cq were observed.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.