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Can I use probes with Iowa Black FQ or Iowa Black RQ on Applied Biosystems Real-Time PCR Systems?
Iowa Black FQ and Iowa Black RQ labeled probes can be used on any PCR instrument. Setup varies between instruments but, generally, if there is an option for picking a dye name for a quencher, pick "non-fluorescent (NFQ)", "none" or "dark quencher". The same setting used for MGB quenchers may be used for Iowa Black dark quenchers.