Processing
PACE™ SNP Genotyping Assays
Cost-effective genotyping assays for low- to high-throughput experiments.
PACE (PCR Allele Competitive Extension) SNP genotyping assays are well-suited for projects that require a reliable, cost-effective approach for SNP and indel identification.
Ordering
- PACE is suitable for high-volume projects, such as plant breeding research [1,2]
- IDT and 3CR Bioscience (UK) have partnered to provide an assay and master mix for your genotyping needs
Learn more about how PACE technology works.
Assays (Allele-specific forward and common reverse primers)
- Step 1—have us design your primers. Contact IDT at PACEdesigns@idtdna.com and provide your SNP coordinates or sequences with identified SNPs. IDT works directly with 3CR, the originators of the PACE assay, to design the primers.
- Step 2—send us your primer designs for synthesis. Order the allele-specific forward and common reverse primers by sending your order details to PACEordering@idtdna.com.
Genotyping Master Mix
Order the PACE Genotyping Master Mix from 3CR by going to https://3crbio.com/products/information/.
Resources
Frequently asked questions
How do I design primers for PACE™ genotyping assays?
Contact us and provide your SNP coordinates or sequences with identified SNPs.
IDT works directly with 3CR Bioscience, the originators of the PACE assay, to design the primers.
How do I order PACE™ primers and PACE Genotyping Master Mix?
Order the allele-specific forward primers and common reverse primer from IDT by contacting us.
Note: To have your primers designed, contact us and provide your SNP coordinates or sequences with identified SNPs. IDT works directly with 3CR Bioscience, the originators of the PACE assay, to design the primers.
Order the PACE Genotyping Master Mix from 3CR Bioscience at https://3crbio.com/products/information/.
References
- Somyong S, Phetchawang P, Bihi AK, et al. A SNP variation in an expansin (EgExp4) gene affects height in oil palm. PeerJ. 2022;10:e13046.
- von Maydell D, Brandes J, Lehnert H, et al. Breeding synthetic varieties in annual caraway: observations on the outcrossing rate in a polycross using a high-throughput genotyping system. Euphytica. 2021.
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