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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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What synthetic arrayed library formats are available from IDT? Are additional customization options possible?
- Table 1 shows available customizations for library design, formulations, and shipping formats.
Product Specifications
| Features | Options |
|---|---|
| Design | Predesigned, custom, user-provided |
| CRISPR systems | Cas9, Cas12a, Cas13, prime editing, and other alternative systems |
| Guaranteed Yield | 0.5 nmol, 2 nmol, 5 nmol, and custom normalized deliverables |
| Cas9 gRNA formats | 2-part cRNA:tracrRNA complex and sgRNA |
| Custom lengths supported | 30-150 nt |
| Chemical modifications | 2'-O-methyl RNA, PS linkages, end-blocking Alt-R modifications |
| Plate types | 96- & 384-well PCR, Deep-well, V-bottom, ECHO, custom options available |
| Formulation options | Multi-guide per well; pooled by gene Arrayed (single gRNA/well) Custom formulations upon request |
| QC | Individual ESI/MS |
| Supporting reagents & functional analysis pipeline (optional) | WT Cas9, HiFi Cas9, Cas12a, and Cas12a Ultra Glycerol-free options available in tubes or plates (ideal for robotics) Electroporation Enhancers rhAmpSeq™ CRISPR Analysis System (NGS-based on-/off-target editing analysis) |
- Don’t see what you’re looking for? We are continually expanding our CRISPR library products and we may have what you need. If you are interested in other chemically modified gRNAs (such as CRISPR on/off systems) targeting any sequence from any species, email our CRISPR experts today to discuss customized solutions for your research at CRISPR@idtdna.com.
Categories:
CRISPR genome editingFor research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.