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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is it possible for the Alt-R® Cas9 Electroporation Enhancer to integrate at the Cas9 cut site?

Our next generation sequencing data demonstrated a very low rate of integration of the enhancer into the target site (less than 1 in 10,000 reads, which is much lower than the insertion rate of random pieces of cellular DNA). Therefore, enhancer integration should not negatively impact experimental outcomes.