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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

In calculating Tms for oligos, whose nearest neighbor values do you use?

We use Santa Lucia's nearest neighbor values. Read more about nearest neighbor analysis in this document: Calculation of Tm for Oligonucleotide Duplexes.

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