SNP genotyping on qPCR platforms: Troubleshooting for amplification and cluster separation
Single-nucleotide polymorphisms (SNPs) provide important information about the biology and evolution of different organisms. SNPs may also help predict an individual’s response to certain drugs, susceptibility to environmental factors, and risk of developing particular diseases. As a result, SNPs with important functional roles often become subjects for high-throughput experiments. In this webinar, Daniel Tsang provides an overview of genotyping using real-time PCR (qPCR) technology, discussing challenges and the means to overcome them. He presents a novel qPCR-based genotyping solution, the rhAmp™ SNP Genotyping System, along with its advantages in genotyping, details on cluster separation, and solutions for improving genotype calling accuracy.