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7 Citations found

The group designed a PrimeTime qPCR LNA Probe targeting a P. armandii SNP sequence.The probe was 24 bases long and contained 6 LNA bases in addition to the 5′ 6-FAM label and 3′ black hole quencher (3BHQ_1). A second PrimeTime qPCR LNA Probe was designed to serve as a blocker probe for species of trees related to the species of interest, Pinus armandii. The probe was also 24 bases long with 6 LNA bases in addition to a 5′ HEX label and a 3′ BHQ.  These reporter dyes (HEX and FAM) can be spectrally resolved from each other because they emit at different wavelengths.

PNA (peptic nucleic acid-locked nucleic acid) clamp Primers and LNA (locked nucleic acid) mutant probes from IDT were used in a PNA-LNA PCR clamp assay for EGFR mutation analysis.

Owczarzy R, You Y, Groth CL, Tataurov AV. (2011) Stability and mismatch discrimination of locked nucleic acid-DNA duplexes. Biochem, 50 : 9352–9367.

New nearest-neighbor parameters correctly forecast the positive and negative effects of LNAs on mismatch discrimination. Specificity is enhanced in a majority of sequences and is dependent on mismatch type and adjacent base pairs.

Ugozzoli LA, Latorra D, et al. (2004) Real-time genotyping with oligonucleotide probes containing locked nucleic acids. Analytical Biochemistry, 324 : 143–152.
McTigue PM, Peterson RJ, Kahn JD. (2004) Sequence-dependent thermodynamic parameters for locked nucleic acid (LNA)-DNA duplex formation. Biochemistry, 43 : 5388–5405.

Improved modified probe design through consideration of modified nucleic acid thermodynamics.

Letertre C, Perelle S, et al. (2003) Evaluation of the performance of LNA and MGB probes in 5′-nuclease assays. Mol Cell Probes, 17 : 307–311.