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5 Citations found

Urban MJ, Both S, et al. (2018) Gold nanocrystal-mediated sliding of doublet DNA origami filaments.. Nature Comm. doi: http://doi.org/10.1038/s41467-018-03882-w

In an example of the evolution of DNA-based nanomachinery, researchers devise 2 anti-parallel DNA origami filaments that undergo step-wise, reversible sliding in opposite directions. Movement is powered by DNA fuels mediated by gold nanocrystals.

Strauss MT, Scheuder F, et al. (2018) Quantifying absolute addressability in DNA origami with molecular resolution.. Nature Commun. doi: http://doi.org/10.1038/s41467-018-04031-z

The researchers address the incorporation and accessiblitiy of DNA strands in DNA origami nanostructures with molecular resolution using DNA-PAINT super resolution microscopy. This method can provide feedback to refine design and assembly of these structures.

Zanacchi FC, Manzo C, et al. (2017) A DNA origami platform for quantifying protein copy number in super-resolution.. Nature Methods. doi: http://doi.org/10.1038/nmeth.4342

The authors use DNA origami with GFP antibodies to provide a system for calibrating fluorophore and antibody labeling efficiency. The method should allow researchers to quantify protein copy number within cells using super-resolution microscopy.

The authors create DNA origami nanostructures that undergo light-induced conformational changes. Two linked 14-helix origami bundles form a chiral object with a tunable angle. A photo-responsive, azobenzene-modified DNA segment is added to the template and upon illumination, is converted to a cis-form, altering the angle of the bound origami bundles.

Taylor MRG, Spirek M, et al. (2016) A polar and nucleotide-dependent mechanism of action for RAD51 paralogs in RAD51 filament remodeling.. Molec Cell. doi: http://doi.org/10.1016/j.molcel.2016.10.020

The authors identify a previously unknown stimulatory mechanism (through RAD51 paralog proteins) for homologous recombination--filament remodeling. They use DNA origami nanostructures to elucidate the mechanism by which RAD51 paralogs enhance homologous recombination.