gBlocks Gene Fragments are used to generate constructs for yeast two-hybrid assays.
The authors have developed a multiplex PCR method that allows for quantitative analysis of T- and B- cell receptor diversity using next generation sequencing. gBlocks Gene Fragments are used as templates for optimization of the multiplex primer mix, a critical step for maintaining quantifiable differences and detecting low level transcripts.
Use of RNase H2–dependent PCR to amplify and detect the tcdB gene in a region of the pathogenicity locus of C. difficile, with unknown sequence variation and insertions/deletions.
Ultramer Oligonucleotides were used to construct calibration standards. Each Ultramer contained a target sequence from one of the transcripts under study. All qPCR assays included a set of 3 Ultramer calibrators.
Use of DsiRNAs to inhibit TNF-ɑ secretion in the study of inflammatory diseases. Data shows greater effectiveness of 27mer DsiRNAs than 21mers.
Bladder cancer cells pre-treated with Dicer-substrate siRNA (DsiRNA) against Ki-67, a DNA-binding protein associated with cell proliferation proliferation, promotes cell cycle arrest and enhances curcumin-induced apoptosis.