α6A and α6B were amplified using IDT PrimeTime qPCR Assays. The assays were composed of primers and double-quenched hydrolysis probes containing the 5′ fluorophore FAM, and ZEN and IABkFQ quenchers. Relative mRNA levels were established by normalization to a pool of cDNA and calculated according to the Pfaffl mathematical model.
The scientists combined reverse transcription with RNase H2-dependent qPCR using blocked cleavable primers to measure levels of alternatively spliced transcripts of latrophilins and of teneurins.
FAM-labeled and Cy5.5-labeled probes were synthesized using standard solid-phase phosphoramidite chemistry and followed by HPLC purification. The Cy5.5-labeled probes included ZEN internal quencher, Iowa Black quenchers or inverted dT on the 3′ ends, and amine on the 5′ ends. Probe identities were confirmed using electron spray ionization mass spectrometry (ESI-MS).
gBlocks Gene Fragments were used to generate codon-optimized cytochrome P450 enzymes, and other hemoproteins, in order to study their catalytic use in non-natural olefin cyclopropanation reactions.
gBlocks Gene Fragments were used to construct a codon optimized Cas9 sequence for expression in Drosophila. Optimization of the sequence was performed using the IDT Codon Optimization tool.
gBlocks Gene Fragments were used to create sgRNA, gene specific sequences.
Researchers at the Foundation for Applied Molecular Evolution (Gainesville, FL, USA) use Ultramer DNA Oligos in experiments demonstrating the practical improvements that can be made to recombinase polymerase amplification (RPA), through addition of self-avoiding molecular recognition systems (SAMRS) to RPA-primers.
gBlocks Gene Fragments were used to generate codon-optimized acRAF genes from <em>H. neapolitanus</em> and <em>P. marinus</em>.
gBlocks Gene Fragments were used as positive genotype controls for the described 5′ nuclease, SNP genotyping assay.