321 Citations found

Zhang X, Zajac AL, Huang L, Behlke MA, Tsourkas A. (2014) Imaging the directed transport of single engineered RNA transcripts in real-time using ratiometric bimolecular beacons. PLoS One, 9 : e85813.
Sarvestani ST, Tate MD, Moffat, JM, Jacobi AM, Behlke, MA, Miller AR, Beckham SA, McCoy CE, Chen W, Mintern JD, O’Keeffe M, John M, Williams BR, Gantier MP. (2014) Inosine-mediated modulation of RNA sensing by Toll-like receptor 7 (TLR7) and TLR8. J Virol, 88 : 799–810.

α6A and α6B were amplified using IDT PrimeTime qPCR Assays. The assays were composed of primers and double-quenched hydrolysis probes containing the 5′ fluorophore FAM, and ZEN and IABkFQ quenchers. Relative mRNA levels were established by normalization to a pool of cDNA and calculated according to the Pfaffl mathematical model.

Dudek H, Wong DH, Arvan R, Shah A, Wortham K, Ying B, Diwanji R, Zhou W, Holmes B, Yang H, Cyr WA, Zhou Y, Shah A, Farkiwala R, Lee M, Li Y, Rettig GR, Collingwood MA, Basu SK, Behlke MA, Brown BD. (2014) Knockdown of β-catenin with Dicer-substrate siRNAs reduces liver tumor burden in vivo. Mol Ther, 22 : 92–101.

The scientists combined reverse transcription with RNase H2-dependent qPCR using blocked cleavable primers to measure levels of alternatively spliced transcripts of latrophilins and of teneurins.

Hernandez FJ, Huang L, et al. (2014) Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe. Nature Med, 20 : 301–306.

FAM-labeled and Cy5.5-labeled probes were synthesized using standard solid-phase phosphoramidite chemistry and followed by HPLC purification. The Cy5.5-labeled probes included ZEN internal quencher, Iowa Black quenchers or inverted dT on the 3′ ends, and amine on the 5′ ends. Probe identities were confirmed using electron spray ionization mass spectrometry (ESI-MS).

Hernandez FJ, Huang L, Olson ME, Power KM, Hernandez LI, Meyerholz DK, Thedens DR, Behlke MA, Horswill AR, JcNamara JO 2nd. (2014) Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe. Nat Med, 20 : 301–306.
Heel T, McIntosh JA, et al. (2014) Non-natural olefin cyclopropanation catalyzed by diverse cytochrome P450s and other hemoproteins. Chembiochem, 15 : 2556–2562.

gBlocks Gene Fragments were used to generate codon-optimized cytochrome P450 enzymes, and other hemoproteins, in order to study their catalytic use in non-natural olefin cyclopropanation reactions.

Port F, Chen HM, et al. (2014) Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci USA, 111 : E2967–2976.

gBlocks Gene Fragments were used to construct a codon optimized Cas9 sequence for expression in Drosophila. Optimization of the sequence was performed using the IDT Codon Optimization tool.

Krishnamurthy S, Behlke MA, Apicella MA, McCray PB, Davidson BL. (2014) Platelet activating factor receptor activation improves siRNA uptake and RNAi responses in well-differentiated airway epithelia. Mol Ther Nucl Acids, 3 : e175.
O'Connell MR, Oakes BL, et al.. (2014) Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature, 516 : 263–266.

gBlocks Gene Fragments were used to create sgRNA, gene specific sequences.

Lindqvist LM, Heinlein M, et al. (2014) Prosurvival Bcl-2 family members affect autophagy only indirectly, by inhibiting Bax and Bak. Proc Natl Acad Sci USA, 111 : 8512–8517.
Song Y, Zhang X, Huang L, Behlke MA, Tsourkas A. (2014) Real-time imaging of single engineered RNA transcripts in living cells using ratiometric bimolecular beacons. J Vis Exp, 90 : e51544.

Researchers at the Foundation for Applied Molecular Evolution (Gainesville, FL, USA) use Ultramer DNA Oligos in experiments demonstrating the practical improvements that can be made to recombinase polymerase amplification (RPA), through addition of self-avoiding molecular recognition systems (SAMRS) to RPA-primers. 

Bestas B, Moreno PM, Blomberg KE, Mohammad DK, Sutlu T, Nordin JA, Guterstam P, Gustafsson MO, Kharazi S, Piatosa B, Roberts TC, Behlke MA, Wood MJ, Gait MJ, Lundin, KE, El Andaloussi S, Mansson R, Berglof A, Wengel J, Smith CI. (2014) Splice-correcting oligonucleotides restore BTK function in X-linked agammaglobulinemia model. J Clin Invest, 124 : 4067–4081.

gBlocks Gene Fragments were used to generate codon-optimized acRAF genes from <em>H. neapolitanus</em> and <em>P. marinus</em>.

Cervinski MA, Sam SS, et al. (2014) Validation of interleukin 28B genotyping assay for clinical use. Clin Biochem, 47 : 478–480.

gBlocks Gene Fragments were used as positive genotype controls for the described 5′ nuclease, SNP genotyping assay. 

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