321 Citations found

Gunawardana M, Chang S, et al. . (2014) Isolation of PCR quality microbial community DNA from heavily contaminated environments.. J Microbiol Methods, 102 : 1–7.

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

Khairuddin N, Blake SJ, Firdaus F, Steptoe RJ, Behlke MA, Hertzog PJ, McMillan NA. (2014) In vivo comparison of local versus systemic delivery of immunostimulating siRNA in HPV-driven tumours. Immunol Cell Biol, 92 : 156–163.

PrimeTime qPCR Assays (primers and probes) for TRAAK, TASK-1, TASK-2, TALK-1, TRESK-2, and GAPDH were used in this study. Experiments were repeated a minimum of three times. The 2ΔΔCt method was used to calculate fold changes in gene expression.

Huang H, Suslov NB, et al. (2014) A G-quadruplex-containing RNA activates fluorescence in a GFP-like fluorophore. Nat Chem Biol, 10 : 686–691.

Real-time PCR was performed using SYBR Green intercalating dye and primers. The primers were designed using the IDT PrimerQuest Primer Design Tool and synthesized by IDT. Relative gene expression (target gene expression normalized to the expression of the endogenous control gene) was calculated using the comparative Ct method (2−ΔΔCt).

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

Target-capture using xGen Lockdown Probes was combined with throughput RNA sequencing to provide high resolution detection of known gene fusions. The targeted gene fusions were known to be associated with several childhood sarcomas.

Kurian AW, Hare EE, et al.. (2014) Clinical evaluation of a multiple-gene sequencing panel for hereditary cancer risk assessment. J Clin Oncol, 32 : 2001–2009.

xGen Lockdown Probes were used to target an entire coding region, exon-intron boundaries, and segments of DNA containing known pathogenic variants in BRCA1 and BRCA2 genes.

Changes in short RNA abundance were tested for their possible influence on mRNA levels of 10 relevant genes. RT-qPCR expression analysis was performed on the 10 genes that displayed clear changes in short RNA read numbers in both sense and antisense orientation and their neighboring genes. cDNA was amplified using PrimeTime qPCR Assays (primers and 5' hydrolysis probes). Expression values represented the ΔCt values compared to β-actin.

Hammond SM, McClorey G, Nordin JZ, Godfrey C, Stenler S, Lennox KA, Smith CE, Jacobi AM, Varela MA, Lee Y, Behlke MA, Wood MJ, Andaloussi SE. (2014) Correlating in vitro splice switching activity with systemic in vivo delivery using novel ZEN-modified oligonucleotides. Mol Ther Nucl Acids, 3 : e212.
Yang L, Yang JL, et al. (2014) CRISPR/Cas9-directed genome editing of cultured cells. Curr Protoc Mol Biol, 107 : 1–17.
Dawes M, Moore-Harrison T, et al. (2014) Differential gene expression in high- and low-active inbred mice. Biomed Res Int, 2014 : 361048.

The expression of 9 genes [Actn2, Actn3, Casq1, Drd2, Lepr, Mc4r, Mstn, Papss2, and Glut4 (a.k.a. Slc2a4)] with putative roles in the regulation of voluntary physical activity was evaluated via RT-qPCR of skeletal muscle and brain RNA from inherently high- and low-active mice. cDNA was amplified using PrimeTime Predesigned qPCR Assays, which included ZEN Double-Quenched Probes, with all reactions run in duplicate. Expression was normalized to an endogenous control (18S ribosomal RNA (IDT assay: RN18S) using methods described by Pfaffl.

Masciarelli S, Fontemaggi G, et al. (2014) Gain-of-function mutant p53 downregulates miR-223 contributing to chemoresistance of cultured tumor cells. Oncogene, 33 : 1601–1608.

Immunoprecipitated DNA was analyzed by real-time PCR using IDT PrimeTime qPCR Assays containing a ZEN Double-Quenched Probes and primers.

Liachko I, Youngblood RA, et al. (2014) GC-rich DNA elements enable replication origin activity in the methylotrophic yeast Pichia pastoris. PLoS Genet, 10 : e1004169.

gBlocks Gene Fragments were used to generate mutant autonomously replicating sequences, or ARSs, associated with replication origins in yeast chromosomal DNA.

Ghorbal M, Gorman M, et al. (2014) Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Nat Biotechnol, 32 : 819–821.

A gBlocks Gene Fragment was used to create an sgRNA expression vector for use with the CRISPR/Cas9 system.

Briner AE, Donohoue PD, et al.. (2014) Guide RNA functional modules direct Cas9 activity and orthogonality. Mol Cell, 56 : 333–339.

gBlocks Gene Fragments were used to create sgRNA expression plasmids. 

Chandra PK, Bao L, et al. (2014) HCV infection selectively impairs type I but not type III IFN signaling. Am J Pathol, 184 : 214–229.

An RT-qPCR assay for HCV quantification was performed with probe-based assays using IDT primers and ZEN double-quenched probes.The assay amplified the 5′ UTR of the HCV genome and provided reduced background and increased signal.

Fei Ye, David C. Samuels, et al.. (2014) High-throughput sequencing in mitochondrial DNA research. Mitochondrion, 17 : 157–160.

The authors explain how next generation sequencing has advanced mitochondrial genetics research. They note how IDT’s xGen Lockdown Probes can be used to accomplish solution phase capture of mtDNA. 

Marbiah MM, Harvey A, et al. (2014) Identification of a gene regulatory network associated with prion replication. EMBO J, 33 : 1527–1547.

5 gBlocks Gene Fragments were used to assemble the ~2 kb coding sequence for Micalcl (NM_027587).

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