321 Citations found

Combining superresolution microscopy with CRISPR-Cas9 genome editing, researchers from Abby Dernburg’s group at UC Berkeley describe a method for building three-dimensional models of the synapsed chromosome axis in C. elegans. Using Alt-R ribonucleoprotein RNP complexes rather than conventional expression plasmids, they report 50-fold improvement of editing efficiency, as measured by the percentage of F1 progeny positive for co-injection markers.

Grahl N, Demers EG, et al.. (2017) Use of RNA-protein complexes for genome editing in non-albicans Candida species . mSphere, 2 : e00218-17.

Researchers at the Geisel School of Medicine at Dartmouth describe an expression-free method of CRISPR-Cas9 genome editing in three non-albicans Candida species using Alt-R Cas9 nuclease and guide RNAs. In this publication, Grahl et al. describe the challenges of using exogenously-expressed Cas9 and gRNAs in these species, and how the use of RNA-protein complexes (ribonucleoprotein) can be used to overcome this obstacle, expanding the potential for CRISPR-Cas9 genome editing to a wider range of fungi species.

The authors create DNA origami nanostructures that undergo light-induced conformational changes. Two linked 14-helix origami bundles form a chiral object with a tunable angle. A photo-responsive, azobenzene-modified DNA segment is added to the template and upon illumination, is converted to a cis-form, altering the angle of the bound origami bundles.

Taylor MRG, Spirek M, et al. (2016) A polar and nucleotide-dependent mechanism of action for RAD51 paralogs in RAD51 filament remodeling.. Molec Cell. doi: http://doi.org/10.1016/j.molcel.2016.10.020

The authors identify a previously unknown stimulatory mechanism (through RAD51 paralog proteins) for homologous recombination--filament remodeling. They use DNA origami nanostructures to elucidate the mechanism by which RAD51 paralogs enhance homologous recombination.

Robertson KA, Hsieh WY, Forster T, Blanc M, Lu H, Crick PJ, Yutuc E, Watterson S, Martin K, Griffiths SJ, Enright AJ, Yamamoto M, Pradeepa MM, Lennox KA, Behlke MA, Talbot S, Haas J, Dölken L, Griffiths WJ, Wang Y, Angulo A, Ghazal P. (2016) An interferon regulated microRNA provides broad cell-intrinsic antiviral immunity through multihit host-directed targeting of the sterol pathway. PLoS Biol, 14 : e1002364.

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

Pépin G, Ferrand J, Höning K, Jayasekara WS, Cain JE, Behlke MA, Gough DJ, G Williams BR, Hornung V, Gantier MP. (2016) Cre-dependent DNA recombination activates a STING-dependent innate immune response. Nucleic Acids Res, 44 : 5356–5364.

This research team used candidate aptamers in an enzyme-linked aptamer sorbent assay (ELASA) to detect human insulin-like growth factor-I, a biomarker for recombinant human growth hormone, used in athletic doping.

Rocha CS, Lundin KE, Behlke MA, Zain R, El Andaloussi S, Smith CI. (2016) Four novel splice-switch reporter cell lines: distinct impact of oligonucleotide chemistry and delivery vector on biological activity. Nucleic Acid Ther, 26 : 381–391.
Donald CL, Brennan B, et al. (2016) Full genome sequence and sfRNA interferon antagonist activity of Zika Virus from Recife, Brazil. PLoS Negl Trop Dis, 10 : e0005048.
Begin-Lavallee V, Midavaine E, Dansereau MA, Tetreault P, Longpre JM, Jacobi AM, Rose SD, Behlke MA, Beaudet N, Sarret P. (2016) Functional inhibition of chemokine receptor CCR2 by dicer-substrate-siRNA prevents pain development. Mol Pain, 12 : 1–16.
Behlke MA, Jacobi AM, Collingwood MA, Schubert MS, Rettig GR, and Turk R . (2016) Genome editing using the Alt-R system with Cas9 protein. Experimental Medicine, Extra Edition (Japan), 34 : 205–210.
Banks JC, Clark JA, Nield P, Staton J-AL, Wagner, E. (2016) Haplotyping cryptic Adélie penguin taxa using low-cost, high-resolution melt curves. New Zea Journ Zool, 43 : 163–170.
Lennox KA, Behlke, MA. (2016) Mini-review on current strategies to knockdown long non-coding RNAs. J Rare Dis Res Treat , 1 : 66–70.
Wright ES, Vetsigian KH. (2016) Quality filtering of Illumina index reads mitigates sample cross-talk. BMC Genomics, 17 : 876.
Zhao D, Yang Y, Qu N, Chen M, Ma Z, Krueger CJ, Behlke MA, Chen AK. (2016) Single-molecule detection and tracking of RNA transcripts in living cells using phosphorothioate-optimized 2′-O-methyl RNA molecular beacons. Biomaterials, 100 : 172–183.
Ramachandran S, Osterhaus SR, Parekh KR, Jacobi AM, Behlke MA, McCray PB Jr. (2016) SYVN1, NEDD8, and FBXO2 regulate δ508-CFTR ubiquitin-mediated proteasomal degradation. J Biol Chem, 291 : 25489–25504.

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

Use of a DNA aptamer to replace monoclonal antibodies in an impedance biosensor that uses microfluidics and a microelectrode for detection of the target molecule, in this case, the H5N1 subtype of avian influenza virus. Aptamer secondary structures and delta G were calculated using the free IDT online UNAFold and OligoAnalyzer programs. Biotinylated aptamers were synthesized by IDT with biotin conjugated at the 5′ end.

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