321 Citations found

Lennox KA, Behlke MA. (2011) Chemical modification and design of anti-miRNA oligonucleotides. Gene Ther, 18 : 1111–1120.
Amen MA, Griffiths A. (2011) Identification and expression analysis of herpes B virus-encoded small RNAs. J Virol, 85 : 7296–7311.

A portion of the second exon of herpes simplex virus, immediate early protein ICP0, was synthesized as a MiniGene synthetic gene. The MiniGene synthetic gene construct was subcloned into a secondary vector system and used to generate RNA for use as a standard in RT-qPCR.

IDT MiniGene constructs were designed as IRF2BP2 (Interferon regulatory factor 2 binding protein 2) mutants: 355 GAC GAC GAC GAC 358 (aspartic acids), TCT 360 TAT (S360A), and TCT 360 GAT (S360D). The MiniGene cosnstructs were flanked by endogenous PstI and NcoI in IRF2BP2 for subcloning by restriction digest.

You Y, Tataurov AV, Owczarzy R. (2011) Measuring thermodynamic details of DNA hybridization using fluorescence. Biopolymers, 95 : 472–486.
Melkman-Zehavi T, Oren R, Kredo-Russo S, Shapira T, Mandelbaum AD, Rivkin N, Nir T, Lennox KA, Behlke MA, Dor Y, Hornstein E. (2011) miRNAs control insulin content in pancreatic β-cells via downregulation of transcriptional repressor. EMBO J, 30 : 835–845.
Melkman-Zehavi T, Oren R, Kredo-Russo S, Shapira T, Mandelbaum AD, Rivkin N, Nir T, Lennox KA, Behlke MA, Dor Y, Hornstein E. (2011) miRNAs control insulin content in pancreatic β-cells via downregulation of transcriptional repressors. EMBO J, 30 : 835–845.
Thiel WH, Bair T, Wyatt-Thiel K, Dassie, JP, Rockey WM, Howell CA, Liu XY, Dupuy AJ, Huang L, Owczarzy R, Behlke MA, McNamara JO, Giangrande PH. (2011) Nucleotide bias observed with a short SELEX RNA aptamer library. Nucleic Acid Ther, 21 : 253–263.

Ultramer oligonucleotides were used to construct a new vector that provides one-step incorporation of the PBAD promoter in front of any gene.

Dobosy JR, Rose SD, Beltz KR, Rupp SM, Powers KM, Behlke MA, Walder JA. (2011) RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers. BMC Biotechnol, 11 : 1–18.
Michel CI, Holley CL, Scruggs BS, Sidhu R, Brookheart RT, Listenberger LL, Behlke MA, Ory DS, Schaffer JE. (2011) Small nucleolar RNAs U32a, U33, and U35a are critical mediators of metabolic stress. Cell Metab, 14 : 33–44.
Owczarzy R, You Y, Groth CL, Tataurov AV. (2011) Stability and mismatch discrimination of locked nucleic acid-DNA duplexes. Biochem, 50 : 9352–9367.

New nearest-neighbor parameters correctly forecast the positive and negative effects of LNAs on mismatch discrimination. Specificity is enhanced in a majority of sequences and is dependent on mismatch type and adjacent base pairs.

Ultramer Oligonucleotides were used as standards due to their purity and quantification precision.

Rockey WM, Huang L, Kloepping KC, Baumhover NJ, Giangrande PH, Schultz MK. (2011) Synthesis and radiolabeling of chelator-RNA aptamer bioconjugates with copper-64 for targeted molecular imaging. Bioorg Med Chem, 19 : 4080–4090.
Lennox KA, Behlke MA. (2010) A direct comparison of anti-microRNA oligonucleotide potency. Pharm. Res, 27 : 1788–1799.
Jodelka FM, Ebert AD, et al.. (2010) A feedback loop regulates splicing of the spinal muscular atrophy-modifying gene, SMN2. Hum Mol Genet, 19 : 4906–4917.

An IDT MiniGene expressing wild-type survival motor neuron from SMN2, and a second SMN2 MiniGene with a silent mutation in the siRNA recognition sequence, were used to study the effects of siRNA knockdown, and rescue, of SMN1/2 on gene splicing. IDT DsiRNA Duplexes were also used for the siRNA knockdown experiments.

Zhou J, Rossi JJ. (2010) Aptamer-targeted cell-specific RNA interference . Silence, 1 : 4.

A review summarizing the use of cell-internalizing aptamers to deliver siRNAs to target cells. Highlights the optimization and improvement of aptamer-targeted siRNAs for clinical translation.

Sarret P, Doré-Savard L, Beaudet N. (2010) Direct application of siRNA for in vivo pain research. Methods Mol Biol, 623 : 383–395.

Dicer-substrate RNA (DsiRNA) is used to target physiological pain modulators in animal models for in vivo pain research.

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