Initiation of isothermal helicase-dependent amplification of a rpoB gene sequence by RNase H2-mediated cleavage of blocked DNA primers.
IDT MiniGene constructs were used as microRNA (miR) sponges against miR-17, miR-18a, miR-19, and miR-92. The sequences were also designed with 5′ Xho1 and 3′ EcoR1 sites for subcloning into other vectors by restriction digest.
Thiel WH, Bair T, Peek AS, Liu X, Dassie J, Stockdale KR, Behlke MA, Miller FJ, Giangrande PH. .
Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection
Dallas A, Ge Q, Ilves H, Kumar P, Shorenstein J, Kazakov SA, Cuellar TL, McManus MT, Behlke MA, Johnston BH.
Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing
Nucleic Acids Res,
Huang YZ, Hernandez FJ, Gu B, Stockdale KR, Nanapaneni K, Scheetz TE, Behlke MA, Peek AS, Bair T, Giangrande PH, McNamara JO..
RNA aptamer-based functional ligands of the neurotrophin receptor, TrkB
Weinstein S, Emmanuel R, Jacobi AM, Abraham A, Behlke MA, Sprague AG, Novobrantseva T, Nagler, A, Peer D.
RNA inhibition highlights Cyclin D1 as a potential therapeutic target for Mantle Cell Lymphoma
Use of DsiRNAs to inhibit viral replication of metapneumovirus in vitro and in vivo. The authors report highly specific inhibition achieved without induction of cytokines or off-target effects.
Walk RM, Elliot ST, Blanco FC, Snyder JA, Jacobi AM, Rose SD, Behlke MA, Salem AK, Vukmanovic S, Sandler AD.
T-cell activation is enhanced by targeting IL-10 cytokine production in toll-like receptor- stimulated macrophages
ImmunoTargets and Therapy,
Ultramer Oligonucleotides were used for BAC mutagenesis via a two-step l–red mediated recombination strategy.
IDT MiniGene synthetic genes were engineered for use in transcription factor binding assays. Mutant sites were created for Sp1, HNF-1, and NF-Y and the MiniGene synthetic genes were provided in pIDTSMART-KAN vectors.
Use of an RNA aptamer–siRNA chimera that triggers sequence-specific degradation of HIV RNAs to suppress HIV-1 replication and prevent CD4+ T cell depletion in humanized mice.
Gantier MP, McCoy CE, Rusinova I, Saulep D, Wang D, Xu D, Irving AT, Behlke MA, Hertzoq PJ, Mackay F, Williams BR.
Analysis of microRNA turnover in mammalian cells following Dicer1 ablation
Nucleic Acids Res,
Ultramer Oligonucleotides were used to generate standard curves for absolute quantification. Each Ultramer standard was designed to contain the 5′-end of a transcript under study and qPCR amplified using transcript-specific primers.