321 Citations found

Rettig GR, Behlke MA. (2012) Progress towards in vivo use of siRNAs - II. Mol Ther, 20 : 483–512.
Lundberg P, Yang H-J, Jung S-J, Behlke MA, Rose SD, Cantin EM. (2012) Protection against TNFα-dependent liver toxicity by intraperitoneal liposome delivered DsiRNA targeting TNFα in vivo. J Control Release, 160 : 194-199.
Ao W, Aldous S, et al. (2012) Rapid detection of rpoB gene mutations conferring rifampin resistance in Mycobacterium tuberculosis. J Clin Microbiol, 50 : 2433–2440.

Initiation of isothermal helicase-dependent amplification of a rpoB gene sequence by RNase H2-mediated cleavage of blocked DNA primers.

Kluiver J, Gibcus JH, et al. (2012) Rapid generation of microRNA sponges for microRNA inhibition. PLoS One, 7 : e29275.

IDT MiniGene constructs were used as microRNA (miR) sponges against miR-17, miR-18a, miR-19, and miR-92. The sequences were also designed with 5′ Xho1 and 3′ EcoR1 sites for subcloning into other vectors by restriction digest.

Thiel WH, Bair T, Peek AS, Liu X, Dassie J, Stockdale KR, Behlke MA, Miller FJ, Giangrande PH. . (2012) Rapid identification of cell-specific, internalizing RNA aptamers with bioinformatics analyses of a cell-based aptamer selection. PLoS One, 7 : e43836.
Dallas A, Ge Q, Ilves H, Kumar P, Shorenstein J, Kazakov SA, Cuellar TL, McManus MT, Behlke MA, Johnston BH. (2012) Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing. Nucleic Acids Res, 40 : 9255–9271.
Huang YZ, Hernandez FJ, Gu B, Stockdale KR, Nanapaneni K, Scheetz TE, Behlke MA, Peek AS, Bair T, Giangrande PH, McNamara JO.. (2012) RNA aptamer-based functional ligands of the neurotrophin receptor, TrkB. Mol Pharmacol, 82 : 623–635.
Weinstein S, Emmanuel R, Jacobi AM, Abraham A, Behlke MA, Sprague AG, Novobrantseva T, Nagler, A, Peer D. (2012) RNA inhibition highlights Cyclin D1 as a potential therapeutic target for Mantle Cell Lymphoma. PLoS ONE, 7 : e43343.

Use of DsiRNAs to inhibit viral replication of metapneumovirus in vitro and in vivo. The authors report highly specific inhibition achieved without induction of cytokines or off-target effects.

Khairuddin N, Gantier MP, Blake SJ, Wu SY, Behlke MA, Williams BR, McMillan NA. (2012) SiRNA-induced immunostimulation through TLR7 promotes antitumoral activity against HPV-driven tumors in vivo. Immunol Cell Biol, 90 : 187–196.
Kim N, Jiang D, Jacobi AM, Lennox KA, Rose SD, Behlke MA, Salem AK. (2012) Synthesis and characterization of mannosylated pegylated polyethylenimine as a carrier for siRNA. Int J Pharm, 427 : 123–133.
Rose S, Behlke MD. (2012) Synthetic dicer-Substrate siRNAs as triggers of RNA interference. In: RNA Interference from Biology to Therapeutics, 1 : 31–56.
Walk RM, Elliot ST, Blanco FC, Snyder JA, Jacobi AM, Rose SD, Behlke MA, Salem AK, Vukmanovic S, Sandler AD. (2012) T-cell activation is enhanced by targeting IL-10 cytokine production in toll-like receptor- stimulated macrophages. ImmunoTargets and Therapy, 1 : 13–23.

Ultramer Oligonucleotides were used for BAC mutagenesis via a two-step l–red mediated recombination strategy.

Heibel SK, Lopez GY, et al. (2012) Transcriptional regulation of N-acetylglutamate synthase. PLoS One, 7 : e29527.

IDT MiniGene synthetic genes were engineered for use in transcription factor binding assays. Mutant sites were created for Sp1, HNF-1, and NF-Y and the MiniGene synthetic genes were provided in pIDTSMART-KAN vectors.

Sakurai K, Amarzguioui M, Kim DH, Alluin J, Heale B, Song MS, Gatignol A, Behlke MA, Rossi JJ. (2011) A role for human Dicer in pre-RISC loading of siRNAs. Nucleic Acids Res, 39 : 1510–1525.

Use of an RNA aptamer–siRNA chimera that triggers sequence-specific degradation of HIV RNAs to suppress HIV-1 replication and prevent CD4+ T cell depletion in humanized mice.

Devor EJ, Huang L, Wise A, Peek AS, Samallow PB. (2011) An X chromosome microRNA cluster in the marsupial species Monodelphis domestica. J Hered, 102 : 577–583.
Gantier MP, McCoy CE, Rusinova I, Saulep D, Wang D, Xu D, Irving AT, Behlke MA, Hertzoq PJ, Mackay F, Williams BR. (2011) Analysis of microRNA turnover in mammalian cells following Dicer1 ablation. Nucleic Acids Res, 39 : 5692–5703.
Flynn RA, Almada AE, Zamudio JR, Sharp PA. (2011) Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome. Proc Natl Acad Sci USA, 108 : 10460–10465.

Ultramer Oligonucleotides were used to generate standard curves for absolute quantification. Each Ultramer standard was designed to contain the 5′-end of a transcript under study and qPCR amplified using transcript-specific primers.

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